Participants/Project Abstracts
Summer 2007 Bioanalytical Science REU Participants
SMALL, ROUND, AND ATTRACTIVE IRON
OXIDE POLYMER BEADS SYNTHESIZED VIA DISPERSION AND SUSPENSION METHODS
Joey Espinosa,
Tierui Zhang, and Professor Yadong Yin
Department
of Chemistry, University of California -Riverside, CA 92521 and University of
California- Santa Barbara, CA 93110
Abstract
Superparamagnetic particles such as iron oxide can be manipulated to move and reorient along a magnetic field without leaving residual charges to clump together when the field is removed. The iron oxide particles traits are highly seen as a possible purification method when combined with a bioconjugate technique. However the iron oxide particles themselves are insufficient to purify such things as protein since they can not attach properly and nor do they dissolve in an aqueous solutions. Thus two experiments were done to embed such iron oxide nanoparticles in a polymer beads. Of course the Iron Oxide nanoparticles were first synthesized by diethyl glycol, oleic acid, and iron Pentacarbonyl reaction. Once the nanoparticles were made two methods known as the dispersion and suspension were used to embed the synthesize nanoparticles. These methods were similar but relied on the hydrophilic and hydrophobic properties to form the beads which also incorporate carboxyl groups that can allow for reactions like amidation that allows the attachment to proteins or antibodies. Once the methods were carried out and the final product were examined it was shown that the optimum beads were synthesized from the dispersal methods that used isopropanol as the solution. The dispersal method showed more uniform and smaller beads in the range of 3-7 microns when examined by a darkfield optical microscope. While the suspension method showed inadequate beads that were 6 times larger and not uniform.
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