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Participants/Project Abstracts

Summer 2006 Bioanalytical Science REU Participants

Zachary Hilton, Rensselaer Polytechnic Institute

LABEL FREE DETECTION OF TARGET BIO-MOLECULES USING A CNT NETWORK FUNCTIONALIZED WITH A CONDUCTING POLYMER

Zachary A. Hilton , Nosang V.Myung, and Ashok Mulchandani
Department of Chemical and Environmental Engineering, University of California-Riverside : Riverside , CA 92521 and Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute: Troy , NY 12180

Label free detection of Bovine Serum Album antigen is obtained by using a functionalized Carbon NanoTube network. CNT create a 1-dimensional Field Effect Transistor which allow for more sensitive biosensor. Observing the current response of a sample, highly selective detection of only a few antigen molecules is performed. It is believed the antibody, entrapped in a polypyrrole membrane, reversibly binds with its corresponding antibody causing a significant change in the resistance of the electrode. This change in resistance is measured in real time by an observable change in the current passing through the electrode. Because binding is reversible, regeneration of the electrode is relatively simple. Electrode fabrication has been optimized showing best results depositing 0.06 uL of a 1 mg/mL CNT solution with a constant applied potential from 1-3V. Polymerization, to entrap the targeted antibody, occurs most readily after the antibody has time to associate with the CNT network and then after a constant potential ranging from 0.6V-1.5V. Sensitivity for the targeted antibody is measured by as a difference in the resistance as the potential swept from -0.5 to 0.5 V, in the presence of a constant voltage.

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